PureScript III Pro Reverse Transcriptase(RTH03-E311)
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Description :PureScript III Pro Reverse Transcriptase expressed in E.coli carrying an improved reverse transcriptase gene cloned from M-MLV. It is an upgraded version of PureScript II Pro Reverse Transcriptase, designed for efficient reverse transcription reactions at 37°C.
Expression System:E.coli
Components :Component Name
PureScript III Pro Reverse Transcriptase
5 × PureScript III Buffer
Storage and Stability :Store product at –30oC to –15oC. For optimal performance aliquot product into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :PureScript III Pro Reverse Transcriptase is a new generation reverse transcriptase optimized from the M-MLV (RNase H ) Reverse Transcriptase. Compared with the previous generation, PureScript III Pro Reverse Transcriptase is designed for efficient reverse transcription reactions at 37oC, while retaining the thermostability of PureScript II Pro. For RNA with complex secondary structure, the reverse transcription reaction temperature can be raised to 50 ~ 55 oC, avoiding the inhibitory effect of complex secondary structure on cDNA synthesis. In addition, this product still has superior continuous synthesis ability and superior impurity tolerance. It is applicable for reverse transcription of animal, plant, and microbial RNA.
References :1. Kotewicz ML, D'Alessio JM, Driftmier KM, Blodgett KP, Gerard GF. Cloning and overexpression of Moloney murine leukemia virus reverse transcriptase in Escherichia coli. Gene. 1985;35(3):249-58. PMID: 2412939.
2. Gerard GF, D'Alessio JM, Kotewicz ML, Noon MC. Influence on stability in Escherichia coli of the carboxy-terminal structure of cloned Moloney murine leukemia virus reverse transcriptase. DNA. 1986 Aug;5(4):271-9. PMID: 2427293.
Specific Activity :The specific activity of PureScript III Pro Reverse Transcriptase was determined to be 200 Units/µl.
Unit Definition:
One unit (U) is defined as the amount of enzyme that incorporates 1 nmol of dTTP into acid-insoluble material in 10 min at 37oC with Poly (rA)-Oligo (dT) as the template/primer.
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