PureScript II Pro Reverse Transcriptase(RTH02-E311)
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Description :PureScript II Pro Reverse Transcriptase expressed in E. coli carrying an improved reverse transcriptase gene cloned from M-MLV.
Species :
Tag :
Expression System:E.coli
Sequence :
Components :Component Name
PureScript II Pro Reverse Transcriptase
5X PureScript II Buffer
Storage and Stability :Store product at –30oC to –15oC. For optimal performance aliquot product into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :PureScript II Pro Reverse Transcriptase is a new generation reverse transcriptase optimized from the M-MLV (RNase H ) Reverse Transcriptase. Compared with the previous generation reverse transcriptase, the thermo-stability of this product is significantly improved. The half-life of PureScript II Pro Reverse Transcriptase at 50oC is >240 min. At 55oC, the half-life of the enzyme is >1 hour, which significantly benefits the transcription of RNA templates with complex secondary structure. In addition, it has an improved template affinity and cDNA synthesis efficiency. It has a good resistance to most RT-PCR inhibitors and is suitable for long-fragment cDNA amplification (up to 20 kb).PureScript II Pro Reverse Transcriptase is applicable for reverse transcription of animal, microbial, and plant RNA rich in polysaccharides and polyphenols.
References :1. Kotewicz ML, D'Alessio JM, Driftmier KM, Blodgett KP, Gerard GF. Cloning and overexpression of Moloney murine leukemia virus reverse transcriptase in Escherichia coli. Gene. 1985;35(3):249-58. doi: 10.1016/0378-1119(85)90003-4. PMID: 2412939.
Specific Activity :The specific activity of PureScript II Pro Reverse Transcriptase was determined to be 200 Units/µl.
Unit Definition:
One unit (U) is defined as the amount of enzyme that incorporates 1 nmol of dTTP into acid-insoluble material in 10 min at 37℃ with Poly (rA)-Oligo (dT) as the template/primer.
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