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RNF34L (CARP2), Active(R297-380G)

RNF34L (CARP2), Active(R297-380G)

  • $215.00


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Description :Recombinant full-length human RNF34L was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full length

Genbank Number :NM_001017368

Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Ubiquitin Enzyme Assay, Western Blot

Molecular Weight :73 kDa

Gene Aliases :FRING; CARP2; CARP-2; RNF189; RNF34L; RIFIFYLIN

Scientific Background :E3 ubiquitin-protein ligase RNF34L, also named CARP2 (Caspases-8/10 Associated RING protein 2), acts as RING‑domain E3 ligases, ubiquitinates apical caspases and targets them for proteasome-mediated degradation. CARP gene silencing inhibits tumor cell survival and increases cancer cell sensitivity to the death ligand or chemotherapy-induced apoptosis. CARP proteins target the tumor suppressor p53 for degradation. RNF34L also acts at the level of endocytic vesicles to limit the intensity of TNF-induced NF-κB activation by the regulated elimination of the adaptor protein RIP1.

References :
1. Liao, W. et al: CARP-2 is an endosome-associated ubiquitin ligase for RIP and regulates TNF-induced NF-kappaB activation. Curr Biol. 18(9):641-649, 2008

2. McDonald, E.R. 3rd et al: Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth. PNAS. 20;101(16):6170-6175, 2004 br>
3. Yang, W. et al: CARPs are E3 ligases that target apical caspases and p53. Cancer Biol. Ther. 6(11):1676-1683, 2007

Product Sheets (By Lot #) :

V2538-5.pdf

Research Areas :Apoptosis/Autophagy, Cancer, Metabolic Disorder, Neurobiology