Rb (C-Term) Protein(R05-55G)
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Description :Recombinant human Rb (773-928) was expressed in E. coli cells using a N-terminal GST tag.
Tag :GST tag
Genbank Number :NM_000321
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~44 kDa
Gene Aliases :RB1, OSRC
Scientific Background :Rb is the retinoblastoma gene product and was the first tumor suppressor cloned. Rb is a negative regulator of the cell cycle through its ability to bind the transcription factor E2F and repress transcription of genes required for S phase (1). Rb can bind to MDM2 and this overcomes both the antiapoptotic function of MDM2 and the MDM2-dependent degradation of p53. Rb associates with the Polycomb group (PcG) proteins to form a repressor complex that blocks entry of cells into mitosis (2). Rb colocalizes with nuclear PcG complexes and is important for association of PcG complexes with nuclear targets.
1. Hanahan, D.et al: The hallmarks of cancer. Cell 100: 57-70, 2000.
2. Dahiya, A. et al: Linking the Rb and Polycomb pathways. Molec. Cell 8: 557-568, 2001.
Product Sheets (By Lot #) :
Research Areas :Cancer, Neurobiology, Cell Cycle, Cancer, Neurobiology, Cell Cycle