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Description :Recombinant human RanBP2 (2553-2838) was expressed in E.coli cells using an N-terminal His tag.
Tag :His tag
Genbank Number :NM_006267
Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Ubiquitin Enzyme Assay, Western Blot
Molecular Weight :~48 kDa
Gene Aliases :ADANE; ANE1; IIAE3; NUP358; TRP1; TRP2
Scientific Background :RNA binding protein 2 (RanBP2) acts an E3 SUMO-protein ligase interacting with E2 ligase UBE2I to facilitate SUMO1 and SUMO2 transferring (1). RanBP2 is involved in the modification of nuclear transport by functioning together with the nuclear pore complex through sumoylation (2). Also, RanBP2 is important for the assembly of the PML by sumo conjugation at ‘Lys-490’ (3). In addition, RanBP2 facilitate the modification of HDAC4 (4).
1. Pichler A., et al. The nucleoporin RanBP2 has SUMO1 E3 ligase activity. Cell, 108:109-120, 2002.
2. Reverter D., et al. Insights into E3 ligase activity revealed by a SUMO-RanGAP1-Ubc9-Nup358 complex. Nature, 435:687-692, 2005.
3. Tatham MH, et al. Unique binding interactions among Ubc9, SUMO and RanBP2 reveal a mechanism for SUMO paralog selection. Nat Struct Mol Biol, Jan;12(1):67-74, 2005.
4. Kirsh O., et al. The SUMO E3 ligase RanBP2 promotes modification of the HDAC4 deacetylase. EMBO J., 21:2682-2691, 2002.
Product Sheets (By Lot #) :
Research Areas :Cancer, Cell Cycle, Inflammation