Anti-RHEB(R42-363BR)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the RHEB protein
Cited Applications :WB, ELISA, IF, IHC
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :RHEB antibody was raised against a 15 amino acid synthetic peptide from the middle region of human RHEB
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of RHEB in mouse brain cell lysate with RHEB antibody at (A) 1, (B) 2, and (C) 4 ug/ml.
Sample Data :Immunohistochemistry of RHEB in mouse brain tissue with RHEB antibody at 2 ug/ml.
Scientific Background :RHEB is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. RHEB is vital in the regulation of growth and cell cycle progression. RHEB has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form. Drosophila RHEB is a direct target of Tsc2 GTPase-activating protein (GAP) activity both in vivo and in vitro. Point mutations in the Drosophila RHEB gene inhibits cell growth while overexpression promotes cell growth. RHEB functions downstream of Tsc1 and Tsc2 in the mTOR signaling pathway.
References :
1. Stocker, H. et al: Rheb is an essential regulator of S6K in controlling cell growth in Drosophila. Nature Cell Biol. 5: 559-565, 2003.
2. Zhang, Y. et al: Rheb is a direct target of the tuberous sclerosis tumour suppressor proteins. Nature Cell Biol. 5: 578-581, 2003.
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Research Areas :Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology, Cancer, Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, Apoptosis/Autophagy, Angiogenesis, Invasion/Metastasis