Anti-Rel A(R29-63BR)

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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the REL A (NFKB/p65) protein

Cited Applications :WB

Cross Reactivity :Western blot human and mouse REL A

REL A from other species may also be detectable.

Host  :Rabbit

Immunogen :Synthetic peptide corresponding to amino acids 527-531 of human REL A

Purification :Affinity chromatography

Stability :Store at 4^oC (add 0.1% NaN₃) for several months, and at -20^oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Representative Western blot with Anti-REL A (1:1000) using 20 ng human recombinant Rel A.

Sample Data :Representative Western blot with Anti-REL A (1:1000) using 3 μg, 10 μg, and 30 μg of 3T6 cell lysate.

Scientific Background :Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses in unstimulated cells, NF-κB is sequestered in the cytoplasm by its inhibitory proteins, the IκBs (1). NF-κB-activating agents can induce the phosphorylation of IκB’s, targeting them for rapid degradation through an ubiquitin-proteasome pathway, releasing NF-κB to enter the nucleus, where it regulates gene expression.

References :
1. Baeuerle, P. et al: Function and activation of NF-kappa B in the immune system. Annu Rev Immunol. 1994; 12: 141-79.

Product Sheets (By Lot #) :

M132-6.pdf

Research Areas :AKT/PKB Pathway, Apoptosis/Autophagy, Inflammation, p38 Pathway, Inflammation, AKT/PKB Pathway, p38 Pathway, Apoptosis/Autophagy