ROS1(L2086F), Active(R14-12CG)

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Description :Recombinant human ROS1 (L2086F) (1881-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST

Expression System:Sf9 insect cells using baculovirus

Sequence :1881-end

Alternative Name(s) :c-ros-1; MCF3; ROS

Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, and 25% glycerol.

Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background :ROS1 is a proto-oncogene and member of the sevenless subfamily of tyrosine kinase insulin receptor genes. ROS1 is highly-expressed in a variety of tumor cell lines and functions as a growth or differentiation factor receptor. The FIG gene can fuse with the ROS1 gene in glioblastoma cell lines (1). The resulting ROS1/FIG fusion protein is a constitutively activated tyrosine kinase. Direct interaction of ROS1 and the phosphatase SHP-1 can lead to efficient downregulation of ROS1-mediated signaling (2). Binding sites in the ROS1 cytoplasmic domain display high affinity binding to the SHP-1 N-terminal SH2 domain. Structural models of ROS1 (L2086F) predict resistance to crizotinib, entrectinib, and lolartanib via steric hindrance (3).

References :1. Charest, A. et al: Fusion of FIG to the receptor tyrosine kinase ROS in a glioblastoma with an interstitial del(6)(q21q21). Genes Chromosomes Cancer 37: 58-71, 2003.
2. Biscup, C. et al: Visualization of SHP-1-target interaction. J Cell Sci. 2004 Oct 1;117(Pt 21):5165-78. 3. Gendarme, S. et al: ROS-1 Fusions in Non-Small-Cell Lung Cancer: Evidence to Date. Curr. Oncol. 29(2):641-658, 2022.

Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet

Purity :Sample Purity. For specific information on a given lot, see related technical data sheet.

Molecular Weight :Observed MW ~82 kDa.
Calculated MW ~79 kDa.

Product Sheets (By Lot #) :

Y4232-11.pdf

Research Areas :Cancer, Cell Cycle, Cellular Stress