Anti-Phospho-ROCK2 (Tyr256)(R11-65R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the human ROCK-2 protein phosphorylated at Tyrosine 256
Cited Applications :ELISA, IHC, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to residues surrounding Tyrosine 256 of human ROCK-2
Purification :Immunoaffinity chromatography
Stability :1yr at –20oC from date of shipment.
Sample Data :Western blot using affinity purified Anto-Phospho-ROCK2 (Tyr256) (1:1,000)shows detection of phosphorylated ROCK-2 in transfected 293T cells. Lane 1 shows lysate expressing exogenous ROCK-2 where the insert contains the transversion Y256A. Lane 2 shows the lysate expressing WT ROCK-2.
Scientific Background :ROCK2 is a ubiquitously expressed serine/threonine kinase localized in the nucleus that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element (1). ROCK2 is an immediate downstream target of the small GTPase RhoA. ROCK2 may play a pivotal role in cardiovascular diseases such as vasospastic angina, ischemic stroke, and heart failure. Inhibition of ROCKs by statins or other selective inhibitors leads to the upregulation and activation of endothelial nitric oxide synthase (eNOS) and reduction of vascular inflammation and atherosclerosis (2).
References :
1. Zhao, Z. et al: Rho-associated kinases play a role in endocardial cell differentiation and migration. Dev Biol. 2004 Nov 1;275(1):183-91.
2. Noma, K. et al: Physiological role of ROCKs in the cardiovascular system. Am J Physiol Cell Physiol. 2006 Mar;290(3):C661-8.
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Research Areas :Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Ser/Thr Kinases