Anti-RIPK1(R07-63R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the RIPK1 protein
Cited Applications :WB, ELISA, IF, IHC
Cross Reactivity :Human, Mouse and Rat
Host / Isotype / Clone# :Rabbit, IgG
Immunogen :RIPK1 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human RIPK1
Purification :Affinity chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot analysis of RIPK1 in rat kidney tissue lysate with RIPK1 antibody at 1 ug/ml.
Sample Data :Immunohistochemistry of RIPK1 in mouse kidney tissue with RIPK1 antibody at 2.5 ug/ml.
Scientific Background :RIPK1 or Receptor Interacting Protein Kinase 1 is a serine/threonine kinase that was originally identified as interacting with the cytoplasmic domain of FAS. RIPK1 has been deemed as an important element in the signal transduction machinery that mediates programmed cell death. RIPK1 has been shown to interact with TRADD, TRAF1 TRAF2 and TRAF3 and TRADD can act as an adaptor protein to recruit RIPK1 to the TNFR1 complex in a TNF-dependent process (1). TNFα is capable of activating the noncanonical NF-κB pathway, but this activation of this pathway is negatively regulated by RIPK1 (2).
References :
1. Hsu, H. et al: TNF-dependent recruitment of the protein kinase RIP to the TNF receptor-1 signaling complex. Immunity 4: 387-396, 1996.
2. Kim, J Y. et al: TNFα induced noncanonical NF-κB activation is attenuated by RIP1 through stabilization of TRAF2. J Cell Sci. 2011 Feb 15;124(Pt 4):647-56.
Product Sheets (By Lot #) :
Research Areas :Ser/Thr Kinases, p38 Pathway, JNK/SAPK Pathway, JAK/STAT Pathway, Inflammation, Cancer, Apoptosis/Autophagy, Cancer, Inflammation, p38 Pathway, JNK/SAPK Pathway, JAK/STAT Pathway, Apoptosis/Autophagy, Ser/Thr Kinases