PTC3 (NCOA4-RET), Active(R02-19DG)

PTC3 (NCOA4-RET), Active(R02-19DG)

  • $259.00


Description :Recombinant human RET/PTC3, the fusion protein [NCOA4 (1-238)-RET (713-end)], was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :NCOA4 (1-238)-RET (713-end)

Genbank Number :BC001562

Genbank Number 2 :NM_020630

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~98 kDa

Gene Aliases :NCOA4: ELE1, RFG, ARA70, PTC3, DKFZp762E1112 RET: CDHF12, RET51, PTC, RET-ELE1; RET/PTC3

References :
1. Santoro M, et al: Molecular characterization of RET/PTC3; a novel rearranged version of the RET proto-oncogene in a human thyroid papillary carcinoma. Oncogene 1994, 9:509-516.

2. Nikiforov YE: RET/PTC rearrangement in thyroid tumors. Endocr Pathol 2002, 13:3-16.

3. Smida J. et al: Distinct frequency of ret rearrangements in papillary thyroid carcinomas of children and adults from Belarus. Int J Cancer 1999, 80:32-38.

Scientific Background :RET/PTC3 is fused of RET and the activating NCOA4 gene by intrachromosomal paracentric inversions in chromo-some 10 (1). Likes RET/PTC1, it is the most frequent RET rearrangements in papillary thyroid carcinoma (PTC) (2), especially in radiation-induced tumours. The RET/PTC3 rearrangements may be typical for radiation-induced childhood PTC with a short latency period (3). The RET/PTC rearrangements also have been shown in benign thyroid lesions, including Hashimoto's thyroiditis (HT).

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Research Areas :Cancer, Cancer, Neurobiology, Metabolic Disorder, Cell Cycle, Cellular Stress