2019nCoV spike protein RBD (E484K)

2019nCoV spike protein RBD (E484K)

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Description: Recombinant 2019-nCoV Spike protein S1 subunit, RBD (E484K) (319-541) was expressed in CHO cells using a C-terminal his tag.

Species: SARS-CoV-2

Tag: HIS tag

Expression System: CHO Cells

Sequence: 319-541

Genbank Number: MN908947

Purity: Sample Purity Data. For specific information on a given lot, see the related technical datasheet.

Storage, Stability, and Shipping: Store product at –70oC. For optimal storage, aliquot targets into smaller quantities after centrifugation and store at the recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight: 39 kDa

Gene Aliases : 2019-nCoV RBD, SARS-CoV-2 spike RBD, novel coronavirus spike RBD, nCov spike RBD.

Scientific Background: The receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein that recognizes the host ACE2 receptor is a major determinant of viral entry and neutralization, and is the most divergent region (1). Substitution of glutamate (E) to Lysine (K) at position 484 (E484K) in the RBD of the spike protein has been reported in the B.1.351 and P.1 lineages. Viruses harboring E484K mutation have reduced susceptibility to human convalescent plasma and vaccinated individuals (2). As these new variants displace the first-wave virus, it is pivotal to evaluate their transmissibility, virulence and their possible tendency to escape antibody neutralization (3).

References : 

1. Lan J, et al: Crystal structure of the 2019-nCov spike receptor-binding domain bound with the ACE2 receptor. bioRxiv. doi: 
2. Jangra S, et al: The E484K mutation in the SARS-CoV-2 spike protein reduces but does not abolish neutralizing activity of human convalescent and post-vaccination sera. . medRxiv [Preprint]. 2021 Jan 29:2021.01.26.21250543
3. Starr TN, et al: Molecular dynamic simulation reveals E484K mutation enhances spike RBD-ACE2 affinity and the combination of E484K, K417N and N501Y mutations (501Y.V2 variant) induces conformational change greater than N501Y mutant alone, potentially resulting in an escape mutant. Cell. 2020, 182(5):1295-1310.


Product Sheets (By Lot #) :

J4038-3.pdf