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PlGF(P829-40N)

PlGF(P829-40N)

  • $193.00


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Description :Recombinant Human PlGF was expressed in E. coli cells.

Species :Human

Tag :

Expression System:E.coli

Sequence :

Genbank Number :P49763

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method

Molecular Weight :15.0 kDa

Gene Aliases :PGF, PGLF

Reconstitution Protocol :A quick spin of the vial followed by reconstitution in PBS to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Scientific Background :Human PlGF, or Placental Growth Factor, is part of the PDGF/VEGF family and contains the 8 cysteine residues conserved in the family. It can be alternately spliced into three different mature forms. PGF is typically found as a disulfide linked dimer which is highly glycosylated. It is a key molecule involved in vasculogenesis and angiogenesis, especially during embryogenesis. It also induces the activation of monocytes, as well as their migration and production of inflammatory cytokines. It is primarily expressed by the placental trophoblast during pregnancy, but is also expressed in villious trophoblasts, decidual cells, erythroblasts, keratinocytes, and somewhat in endothelial cells. PGF signals by binding VEGF R1, and competes with VEGF for binding. Recombinant PlGF is a 15.0 kDa protein.

Sample Cell Assay Data :

Purity :Recombinant Human PIGF resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250

Product Sheets (By Lot #) :

G3223-6.pdf

Research Areas :Cancer