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PKN3/PRK3, Active(P69-10G)

PKN3/PRK3, Active(P69-10G)

  • $226.00


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Description :Recombinant full-length human PKN3/PRK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :NM_013355

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~130 kDa

Gene Aliases :RP11-545E17.1

Scientific Background :PKN3 or PRK3 is a serine/threonine protein kinase which can associate with RHOA in a GTP-dependent manner. PKN3 can also interact with GRAF and GRAF2 via the SH3 domains and phosphorylate these proteins. PKN3 is a mediator of malignant growth of human prostate cancer cells and functions downstream of activated PI3K. PKN3 is upregulated in human tumor cells but not in surrounding nontumorigenic tissues. Inducible knockdown of PKN3 expression inhibited metastasis in a mouse prostate tumor model.

References :
1. Shibata, H. et al: PKN-beta interacts with the SH3 domains of Graf and a novel Graf related protein, Graf2, which are GTPase activating proteins for Rho family. J. Biochem. 130: 23-31, 2001.

2. Leenders, F. et al: PKN3 is required for malignant prostate cell growth downstream of activated PI 3-kinase. EMBO J. 23: 3303-3313, 2004.

Product Sheets (By Lot #) :

I123-10.pdf

S069-1.pdf

Y886-3.pdf

K1853-5.pdf

P1687-4.pdf

B1976-3.pdf

L2149-11.pdf

N3518-8.pdf

Research Areas :Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, NfkB Pathway, PKA/PKC Pathway, Ser/Thr Kinases, Neurobiology, Cardiovascular Disease, ERK/MAPK Pathway, NfkB Pathway, PKA/PKC Pathway, Apoptosis/Autophagy, Ser/Thr Kinases