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Description :Recombinant full-length human PI4KA was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_002650
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay
Molecular Weight :~120 kDa
Gene Aliases :FLJ16556; PI4K-ALPHA; pi4K230; PIK4CA
Scientific Background :PI4KA encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by PI4KA gene is a type III enzyme that is not inhibited by adenosine. PI4KA is highly expressed in placenta and brain (1). PI4KA is associated with the membranes of Golgi vesicles and vacuoles (2).
1. Wong, K. et.al: Cloning and characterization of a human phosphatidylinositol 4-kinase. J. Biol. Chem. 269: 28878-28884, 1994.
2. Nakagawa, T. et.al: Cloning, expression, and localization of 230-kDa phosphatidylinositol 4-kinase. J. Biol. Chem. 271: 12088-12094, 1996.
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Research Areas :AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Lipid Kinases, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling