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PI3K (p110 beta/p85 beta), Active(P28-102BH)

PI3K (p110 beta/p85 beta), Active(P28-102BH)

  • $259.00


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Description :Recombinant full-length human p110 beta and human p85 beta were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.

Species :Human

Tag :His tags

Expression System:Sf9 insect cells using baculovirus

Sequence :Full length

Genbank Number :NM_006219

Genbank Number 2 :NM_005027

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay

Molecular Weight :p110beta ~111 kDa and p85beta ~88 kDa

Gene Aliases :p110beta: PI3K, PIK3C1, PI3Kbeta, MGC133043, p110-BETA
p85beta: PIK3R2, P85B, p85-BETA

Scientific Background :PI3K phosphorylate the 3-prime OH position of the inositol ring of inositol lipids which functions in signaling pathways that regulate cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kDa catalytic subunit and an 85-kDa adaptor subunit (1). PI3K plays a critical role in regulating the formation and stability of alpha-2B-beta-3 integrin adhesion bonds, which are necessary for shear force-induced platelet activation. PI3K sustains alpha-2B-beta-3 integrin activation and stabilized platelet aggregation by regulating both integrin-dependent calcium flux and Gi activation of RAP1B (2).

References :
1. Hu, P.et.al: Cloning of a novel, ubiquitously expressed human phosphatidylinositol 3-kinase and identification of its binding site on p85. Molec. Cell. Biol. 13: 7677-7688, 1993.

2. ackson, S. P.et.al: PI 3-kinase p110-beta: a new target for antithrombotic therapy. Nature Med. 11: 507-514, 2005.

Product Sheets (By Lot #) :

L2098-7.pdf

Research Areas :AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Lipid Kinases, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling