PI3K (p110 beta/p85 beta), Active(P28-102BH)
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Description :Recombinant full-length human p110 beta and human p85 beta were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Tag :His tags
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_006219
Genbank Number 2 :NM_005027
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay
Molecular Weight :p110beta ~111 kDa and p85beta ~88 kDa
Gene Aliases :p110beta: PI3K, PIK3C1, PI3Kbeta, MGC133043, p110-BETA
p85beta: PIK3R2, P85B, p85-BETA
Scientific Background :PI3K phosphorylate the 3-prime OH position of the inositol ring of inositol lipids which functions in signaling pathways that regulate cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kDa catalytic subunit and an 85-kDa adaptor subunit (1). PI3K plays a critical role in regulating the formation and stability of alpha-2B-beta-3 integrin adhesion bonds, which are necessary for shear force-induced platelet activation. PI3K sustains alpha-2B-beta-3 integrin activation and stabilized platelet aggregation by regulating both integrin-dependent calcium flux and Gi activation of RAP1B (2).
1. Hu, P.et.al: Cloning of a novel, ubiquitously expressed human phosphatidylinositol 3-kinase and identification of its binding site on p85. Molec. Cell. Biol. 13: 7677-7688, 1993.
2. ackson, S. P.et.al: PI 3-kinase p110-beta: a new target for antithrombotic therapy. Nature Med. 11: 507-514, 2005.
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Research Areas :AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Lipid Kinases, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling