Due to Easter holiday, all delivery service requests will not be processed on Friday, April 10, 2020. Regular delivery schedule will resume on Monday, April 13, 2019.
PI3K (p110 delta (E1021K)/p85 alpha), Active(P30-122H)

PI3K (p110 delta (E1021K)/p85 alpha), Active(P30-122H)

  • $259.00


FOR BULK ORDER REQUESTS PLEASE CONTACT US

Description :Recombinant full-length human p110 delta (E1021K) and human p85 alpha were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.

Species :Human

Tag :His tags

Expression System:Sf9 insect cells using baculovirus

Sequence :Full-length

Genbank Number :p110 delta: NM_005026

Genbank Number 2 :p85 alpha: NM_181523

Specific Activity : Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping  :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :P110 delta: 111 kDa and p85 alpha: 86 kDa

Gene Aliases :p110 alpha: p110 delta, p85 alpha: PIK3R1, GRB1, p85-ALPHA

Scientific Background :PI3K (p110 delta /p85 alpha) is a member of the PI3K family that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring (1). PI3K (p110delta/p85alpha) plays a role in oncogenic transformation and is consistently expressed at a high level in blast cells from AML. PI3K (p110delta/p85alpha) can play a selective role in the amplification of phosphatidylinositol (3,4,5) trisphosphate (PIP3) levels that lead to neutrophil polarization, and regulates directional migration, but not random locomotion (2). PI3K (p110delta/p85alpha) signaling pathway is involved in immune responses relevant to the pathogenesis of rheumatoid arthritis and other inflammatory diseases.

References :
1. Vanhaesebroeck, B. et.al: p110-delta, a novel phosphoinositide 3-kinase in leukocytes. Proc. Nat. Acad. Sci. 94: 4330-4335, 1997.
2. Chantry, D. et.al: p110-delta, a novel phosphatidylinositol 3-kinase catalytic subunit that associates with p85 and is expressed predominantly in leukocytes. J. Biol. Chem. 272: 19236-19241,1997.

Product Sheets (By Lot #) :

V2396-3.pdf

Research Areas :Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases