PI3K (p110 beta/p85 alpha), Active(P28-102H)
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Description :Recombinant full-length human p110 beta and human p85 alpha were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Tag :His tags
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_006219
Genbank Number 2 :NM_181523
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay
Molecular Weight :p110 beta ~111 kDa and p85 alpha ~86 kDa
Gene Aliases :p110 beta: PIK3CB, PI3K, PIK3C1, PI3Kbeta, p110-BETA, MGC133043, DKFZp779K1237
p85 alpha: PIK3R1, GRB1, p85-ALPHA
Scientific Background :The PI3K comprises of a 110 kDa catalytic subunit and an 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110 beta catalytic subunit (PIK3CB) plays a role in regulating the formation and stability of alpha-2B-beta-3 integrin adhesion bonds, which are necessary for shear force-induced platelet activation (1). In animal model of prostate tumor formation induced by the tumor suppressor PTEN loss, ablation of p110 beta impedes tumorigenesis with a concomitant diminution of AKT phosphorylation (2).
1. Jackson, S. P. et al: PI 3-kinase p110-beta: a new target for antithrombotic therapy. Nature Med. 11: 507-514, 2005.
2. Jia, S.et al: Essential roles of PI(3)K-p110-beta in cell growth, metabolism and tumorigenesis. Nature 454: 776-779, 2008.
Product Sheets (By Lot #) :
Research Areas :AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Lipid Kinases, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling