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PI3K (p110 alpha (N345K)/p85 alpha), Active(P27-122CH)

PI3K (p110 alpha (N345K)/p85 alpha), Active(P27-122CH)

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Description :Recombinant full-length human p110 alpha (N345K) mutant subunit and human p85 alpha wild-type subunit were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.

Species :Human

Tag :His tags

Expression System:Sf9 insect cells using baculovirus

Sequence :Full length

Genbank Number :NM_006218

Genbank Number 2 :NM_181523

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay

Molecular Weight :p110 alpha(N345K) ~111 kDa and p85 alpha ~86 kDa

Gene Aliases :p110 alpha: PIK3CA, MGC142161, MGC142163, p110-alpha
p85 alpha: PIK3R1, GRB1, p85-ALPHA

Scientific Background :The PI3K comprises of a 110 kDa catalytic subunit and an 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110 alpha catalytic subunit (PIK3CA) is frequently mutated or amplified in a variety of cancers including ovarian and colon and this protein is one of the PI3K mutants (1). PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of p110 alpha is essential for vascular development and inactivation of p110 alpha leads to severe defects in angiogenic sprouting and vascular remodeling (2).

References :
1. Samuels, Y. et al: High frequency of mutations of the PIK3CA gene in human cancers. Science 304: 554 only, 2004.

2. Graupera, M.et al: Angiogenesis selectively requires the p110-alpha isoform of PI3K to control endothelial cell migration. Nature 453: 662-666, 2008.

Product Sheets (By Lot #) :

G1249-1B.pdf

Research Areas :AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Lipid Kinases, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling