PI3K (p110 alpha (E545K)/p85 alpha), Active(P27-152H)
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Description :Recombinant full-length human p110 alpha (E545K) mutant subunit and human p85 alpha wild-type subunit were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Tag :His tags
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_006218
Genbank Number 2 :NM_181523
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay
Molecular Weight :p110 alpha(E545K) ~111 kDa and p85 alpha ~86 kDa
Gene Aliases :p110 alpha: PIK3CA, MGC142161, MGC142163, p110-alpha
p85 alpha: PIK3R1, GRB1, p85-ALPHA
Scientific Background :The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110 alpha catalytic subunit (PIK3CA) is frequently mutated or amplified in a variety of cancers including ovarian and colon and this protein is one of the PI3K mutants (1). PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of p110 alpha is essential for vascular development and inactivation of p110 alpha leads to severe defects in angiogenic sprouting and vascular remodeling (2).
1. Samuels, Y. et al: High frequency of mutations of the PIK3CA gene in human cancers. Science 304: 554 only, 2004.
2. Graupera, M.et al: Angiogenesis selectively requires the p110-alpha isoform of PI3K to control endothelial cell migration. Nature 453: 662-666, 2008.
Product Sheets (By Lot #) :
Research Areas :AKT/PKB Pathway, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Inflammation, Invasion/Metastasis, Lipid Kinases, Metabolic Disorder, Neurobiology, NfkB Pathway, WNT Signaling