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Persephin (PSPN)(P870-40N)

Persephin (PSPN)(P870-40N)

  • $193.00


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Description :Recombinant Human Persephin (PSPN) was expressed in E. coli cells.

Species :Human

Tag :

Expression System:E.coli

Sequence :

Genbank Number :O60542

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method

Molecular Weight :10.7 kDa

Reconstitution Protocol :Reconstitute in sterile 4mM HCl at 100μg/mL (containing at least 0.1% human or bovine serum albumin)

Scientific Background :Persephin is a disulfide-linked homodimeric neurotrophic factor belonging to the Transforming Growth Factor-beta (TGF-beta) superfamily and the GDNF sub-family. One cysteine residue, out of the seven conserved, is used for inter-chain disulfide bridging while the others form a cysteine knot ring formation. It signals through RET and one of the four GFR-alpha (alpha-1, alpha-2, alpha-3, alpha-4) receptors to promote the proper development and maintenance of the nervous system. Persephin promotes survival of dopaminergic neurons in the midbrain as well as some motor neurons, and promotes branching of ureteric buds.

Sample Cell Assay Data :The ED50 is 4.0-16.0 ng/mL as determined by a cell proliferation assay.

Purity :Recombinant Human Persephin (PSPN) resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.

Product Sheets (By Lot #) :

G3223-5.pdf

Research Areas :Cancer