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Description :Recombinant human PDE4D (225-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Genbank Number :NM_006203
Specific Activity :Sample Phosphodiesterase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :PDE Assay
Molecular Weight :~82 kDa
Gene Aliases :DPDE3; STRK1; HSPDE4D; PDE4DN2
Scientific Background :PDE4D is a member of the phosphodiesterase (PDE) family that is cAMP-specific and has homology to the 'dunce' gene of Drosophila. PDE4D is expressed in both cytosolic and particulate fractions in the cell and Rolipram, a specific PDE4 inhibitor, inhibits and significantly lowers the IC50 for the cytosolic forms of the enzyme. TNFα upregulates the basal expression of PDE4D in HUVEC. PDE4D, which is not detected in untreated cells, accumulates beginning 4 hours after TNFα treatment and increased by 24 hours (1). Mice deficient in PDE4D exhibit delayed growth as well as reduced viability and female fertility (2).
1. Miro X, et al: Phosphodiesterases 4D and 7A splice variants in the response of HUVEC cells to TNF-alpha. Biochem. Biophys. Res. Commun. 274: 415-421, 2000.
2. Jin S-L C, et al: Impaired growth and fertility of cAMP-specific phosphodiesterase PDE4D-deficient mice. Proc. Nat. Acad. Sci. 96: 11998-12003, 1999.
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Research Areas :Inflammation, Cardiovascular Disease, Phosphodiesterases, Inflammation, Cardiovascular Disease, Phosphodiesterases