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Description :Recombinant human PDE4A (332-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Genbank Number :NM_001111307
Specific Activity :Sample Phosphodiesterase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :PDE Assay
Molecular Weight :~110 kDa
Gene Aliases :PDE4; DPDE2; PDE46
Scientific Background :PDE4A is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP. In vitro phosphorylation of PDE4A by the PKA-catalytic subunit increases the enzyme's sensitivity to Mg(2+), leading to a 4-fold increase in cAMP hydrolysis without affecting the Km. PDE4 is widely expressed in brain tumors and promotes their growth and treatment with PDE4A inhibitor Rolipram overcomes tumor resistance and mediates tumor regression (1). PDE4A expression is altered in patients with bipolar disorder and is a new therapeutic avenue for treatment of this disorder (2).
1. Goldhoff P, et al: Targeted inhibition of cyclic AMP phosphodiesterase-4 promotes brain tumor regression. Clin Cancer Res. 2008 Dec 1;14(23):7717-25.
2. Fatemi S H, et al: Phosphodiesterase-4A expression is reduced in cerebella of patients with bipolar disorder. Psychiatr Genet. 2008 Dec;18(6):282-8.
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Research Areas :Inflammation, Cardiovascular Disease, Phosphodiesterases, Inflammation, Cardiovascular Disease, Phosphodiesterases