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PDE1A, Active(P89-30H)

PDE1A, Active(P89-30H)

  • $259.00


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Description :Recombinant full-length human PDE1A was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Species :Human

Tag :HIS tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :NM_005019

Specific Activity :Sample Phosphodiesterase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :PDE Assay

Molecular Weight :~64 kDa

Gene Aliases :HCAM1; HSPDE1A; MGC26303

Scientific Background :PDE1A is a member of the cyclic nucleotide phosphodiesterase family that is stimulated by Ca2+-calmodulin. PDE1A is a cGMP-specific phosphodiesterase and hydrolyzes cGMP to 5'-GMP. Calmodulin is tightly bound to PDE1A in human spermatozoa and this results in permanent activation of PDE1A (1). Inhibition of PDE1A function significantly attenuates vascular smooth muscle cell growth by decreasing proliferation via G1 arrest and induces apoptosis (2). In vascular smooth muscle cell, PDE1A is important in regulating growth and survival of these cells and thus can contribute to the neointima formation in atherosclerosis and restenosis.

References :
1. Lefièvre L, et al: Presence of cyclic nucleotide phosphodiesterases PDE1A, existing as a stable complex with calmodulin, and PDE3A in human spermatozoa. Biol Reprod. 2002 Aug;67(2):423-30.

2. Nagel D J, et al: Role of nuclear Ca2+/calmodulin-stimulated phosphodiesterase 1A in vascular smooth muscle cell growth and survival. Circ Res. 2006 Mar 31;98(6):777-84.

Product Sheets (By Lot #) :

H079-3.pdf

R148-1.pdf

B2073-10.pdf

Research Areas :Inflammation, Cardiovascular Disease, Phosphodiesterases, Inflammation, Cardiovascular Disease, Phosphodiesterases