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Description :Recombinant human mature form of PCMT1 (2-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Genbank Number :BC007501
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Methyltransferase Assay
Molecular Weight :51 kDa
Gene Aliases :None
Scientific Background :PCMT1 is a member of the L-isoaspartyl/D-aspartyl protein methyltransferase family, which plays an important role in protein repair (1). Downregulation of PCMT1 leads to hyperactivation of MAPK pathway upon EGF stimulation (2). PCMT1 is regulated by cell-matrix interactions, and linked to various biological processes including wound healing, cell migration, and tumor metastasis (3).
1. Tsai, W., et al. Amino acid polymorphisms of the human L-isoaspartyl/D-aspartyl methyltransferase involved in protein repair. Biochem Biophys Res Commun. 203(1):491-7, 1994.
2. Kosugi, S., et al. Suppression of protein l-isoaspartyl (d-aspartyl) methyltransferase results in hyperactivation of EGF-stimulated MEK-ERK signaling in cultured mammalian cells. Biochem Biophys Res Commun. 371(1):22-7, 2008.
3. Lanthier, J., et al. Regulation of protein L-isoaspartyl methyltransferase by cell-matrix interactions: involvement of integrin alphavbeta3, PI 3-kinase, and the proteasome. Biochem Cell Biol. 84(5):684-94, 2006.
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Research Areas :Angiogenesis, Cancer, Cardiovascular Disease, Metabolic Disorder, Neurobiology