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Description :Recombinant human PCAF (431-end) was expressed in E. coli cells using a N-terminal GST tag.
Tag :GST tag
Genbank Number :NM_003884
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Western Blot
Molecular Weight :~69 kDa
Gene Aliases :CAF, GCN5, GCN5L, P/CAF, GCN5L1
Scientific Background :PCAF is a protein that shows homology to the yeast GCN5 and associates with p300/CBP. PCAF has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP (1). PCAF encodes a predicted 832-amino acid protein that showed by RNA blotting to be expressed in all tissues, most strongly in heart and skeletal muscle. PCAF has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. PCAF is required for MyoD activity and muscle differentiation. PCAF directly acetylated MyoD which then displays an increased affinity for its DNA target (2).
1. Yang, X J. et al: A p300/CBP-associated factor that competes with the adenoviral oncoprotein E1A. Nature 382: 319-324, 1996.
2. Sartorelli, V. et al: Acetylation of MyoD directed by PCAF is necessary for the execution of the muscle program. Molec. Cell 4: 725-734, 1999.
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Research Areas :NfkB Pathway, Cell Cycle, Cancer, Cancer, NfkB Pathway, Cell Cycle