PAD4 Protein (P312-30DG)
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Description : Recombinant full-length human PAD4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species : Human
Tag : GST tag
Expression System : Sf9 insect cells using baculovirus
Sequence : Full length
Genbank Number : BC025718
Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications : Western Blot
Molecular Weight : ~96 kDa
Gene Aliases : PADI4; PAD; PDI4; PDI5
Scientific Background : PAD4 is a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions (1). PAD4 is essential for antibacterial innate immunity mediated by neutrophil extracellular traps (NETs) (2). PAD4 play a role in granulocyte and macrophage development leading to inflammation and immune response. PAD4 also mediates the gene expression by regulating arginine methylation and citrullination in histones(3).
1. Nakashima, K. et.al: Molecular characterization of peptidylarginine deiminase in HL-60 cells induced by retinoic acid and 1-alpha,25-dihydroxyvitamin D(3). J. Biol. Chem. 274: 27786-27792, 1999.
2. Li, P.et.al: PAD4 is essential for antibacterial innate immunity mediated by neutrophil extracellular traps. J. Exp. Med. 207: 1853-1862, 2010.
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Research Areas : Inflammation,