PRKG2, Active(P79-10G)
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Description :Recombinant full-length human PRKG2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full Length
Genbank Number :NM_006259
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet. 
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~112 kDa
Gene Aliases :cGKII, PRKGR2
Scientific Background :PRKG2 is a member of the cGMP-dependent protein kinase family and is highly expressed in brain, lung, and intestinal mucosa. PRKG2 mediates intestinal secretion of water and electrolytes induced by the E. coli toxin STa and the intestinal peptide guanylin (1). Mice deficient in PRKG2 are resistant to E. coli STa and developed dwarfism which is caused by a severe defect in endochondral ossification at the growth plates. PRKG2 phosphorylates SOX9 and attenuates SOX9 function by inhibiting its nuclear entry. PRKG2 is a molecular switch that couples the cessation of proliferation and the start of hypertrophic chondrocyte differentiation through attenuating SOX9 function (2).
References :
1. Pfeifer, A. et al: Intestinal secretory defects and dwarfism in mice lacking cGMP-dependent protein kinase II. Science 274: 2082-2084, 1996.
2. Chikuda, H. Et al: Cyclic GMP-dependent protein kinase II is a molecular switch from proliferation to hypertrophic differentiation of chondrocytes. Genes Dev. 18: 2418-2429, 2004.
Product Sheets (By Lot #) :
Research Areas :Cardiovascular Disease, Neurobiology, NfkB Pathway, PKA/PKC Pathway, Ser/Thr Kinases, Neurobiology, Cardiovascular Disease, NfkB Pathway, PKA/PKC Pathway, Ser/Thr Kinases