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Anti-PKC delta/theta(P64-63DR)

Anti-PKC delta/theta(P64-63DR)

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Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the PKCδ/θ protein

Cited Applications :WB

Cross Reactivity :Western blot human PKCdelta/theta

PKCdelta/theta from other species may also be detectable.

Host :Rabbit

Immunogen :Synthesized peptide derived from C-terminus of human PKCθ protein

Purification :Affinity Chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Representative Western blot with Anti-PKCδ/θ (1:1000) using 15ng of GST-tagged Human PKCα, PKCβ І, PKCβ ІІ, PKCγ, PKCδ, PKCε, PKCη, PKCθ, PKCμ, PKCν, PKCζ and PKCι recombinant proteins.

Sample Data :Representative Western blot with PKCδ/θ(1:1000) using 20μg, 40μg, 60μg of Jurkat cell lysate.

Scientific Background :Protein kinase C delta (PKCδ) is a member of the protein kinase C (PKC) family of serine-threonine kinases. It is a 79 kDa protein kinase that shows strict dependence on the presence of phospholipids, but shows no activation by Ca2+ (1). Protein kinase C theta (PKCθ) is important component in the intracellular signaling cascade (2). Recent studies have suggested that local accumulation of fat metabolites inside skeletal muscle may activate a serine kinase cascade involving PKC-theta leading to defects in insulin signaling and glucose transport in skeletal muscle (3).

References :
1. Leibersperger, H. et al: Immunological demonstration of a calcium-unresponsive protein kinase C of the delta-type in different species and murine tissues. Predominance in epidermis. J Biol Chem. 1991 Aug 5; 266(22):14778-84.

2. Manicassamy, S. and Sun, Z. The critical role of protein kinase C-theta in Fas/Fas ligand-mediated apoptosis. J. Immunol. 2007; 178(1):312-9.

3. Kim, J K. et al: PKC-theta knockout mice are protected from fat-induced insulin resistance. J. Clin. Invest. 2004; 114(6):823-7.

Product Sheets (By Lot #) :

M191-1.pdf

Research Areas :Apoptosis/Autophagy, Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, NfkB Pathway, PKA/PKC Pathway, Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, ERK/MAPK Pathway, NfkB Pathway, PKA/PKC Pathway, Apoptosis/Autophagy, Invasion/Metastasis