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Description :Recombinant full-length human p53 was expressed in E. coli cells using an N-terminal GST tag.
Tag :GST tag
Sequence :Full Length
Genbank Number :NM_000546
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay, Western Blot
Molecular Weight :~79 kDa
Gene Aliases :TP53, LFS1, TRP53
Scientific Background :p53 is a key regulator of cell growth and acts as a tumor suppressor gene. Wild-type p53 gene can suppress transformation of rat embryo fibroblasts in cell culture by other oncogenes such as the adenovirus E1A and Ras (1). DNA tumor viruses such as SV40 large T antigen and the adenovirus E1A plus E1B-55Kd proteins bind to p53 and inactivate its tumor suppressor activities leading to cellular transformation. Mutational inactivation of the p53 gene is detected in more than 50% of human cancers. Mutation of p53 renders cancer cells more resistant to current cancer therapies due to lack of p53-mediated apoptosis (2).
1. Levine, A J. et al: Tumor suppressor genes: the p53 and retinoblastoma sensitivity genes and gene products. Biochim Biophys Acta. 1990 Jun 1;1032(1):119-36.
2. Sun, Y. p53 and its downstream proteins as molecular targets of cancer. Mol Carcinog. 2006 Jun;45(6):409-15.
Product Sheets (By Lot #) :
Research Areas :Cancer, Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, p38 Pathway, JNK/SAPK Pathway, Apoptosis/Autophagy, Cell Cycle, Cellular Stress, Cancer, Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, p38 Pathway, JNK/SAPK Pathway, Apoptosis/Autophagy, Cell Cycle, Cellular Stress