Anti-PKAc(P51-63R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the PKAcα, PKAcβ and PKAcγ protein
Cited Applications :WB
Cross Reactivity :Western blot human PKAca, PKAcB and PKAcy
PKAca, PKAcB and PKAcy from other species may also be detectable.
Host :Rabbit
Immunogen :Protein Code: P17612
Purification :Affinity purified using Protein G
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Western Blot :
Representative western blot with Anti-PKAc (1:250) using 20ng of PKAcα, PKAcβ and PKAcγ recombinant protein.wb_gel.jpg)
Scientific Background :The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kinase (PKA or cAPK) in mammalian cells and controls many cellular mechanismssuch as gene transcription, ion transport and protein phosphorylation (1). Inactive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer, and in this inactive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms (-cα, -cβand -cγ) and two families of regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified. Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released.
References :
1. Montminy M: Transcriptional regulation by cyclic AMP. Annu Rev Biochem, 1997;66:807-22.
Product Sheets (By Lot #) :
Research Areas :Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, ERK/MAPK Pathway, NfkB Pathway, PKA/PKC Pathway, Apoptosis/Autophagy, Invasion/Metastasis, Neurobiology, Inflammation, Metabolic Disorder, Cardiovascular Disease, ERK/MAPK Pathway, NfkB Pathway, PKA/PKC Pathway, Apoptosis/Autophagy, Invasion/Metastasis