PLK3, Active(P43-13H)

PLK3, Active(P43-13H)

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Description :Recombinant human PLK3 (57-340) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Species :Human

Tag :HIS

Expression System:Sf9 insect cells using baculovirus

Sequence :57-340

Genbank Number :NM_004073

Alternative Name(s) :CNK, FNK, PRK

Formulation :Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.

Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background :PLK3 or Polo-like kinase 3 is a cytokine-inducible kinase that is a member of the Polo family of serine/threonine protein kinases. PLK3 contains both a catalytic domain and a putative regulatory domain and plays an important role in the regulation of cell cycle progression and tumorigenesis (1). PLK3 is closely associated with centrosomes in several human cell lines and its localization is dependent on the integrity of microtubules. Hence, PLK3 is also involved in regulating microtubule dynamics and centrosomal function (2).

References :1. Li, B.et.al: Prk, a cytokine-inducible human protein serine/threonine kinase whose expression appears to be down-regulated in lung carcinomas. J. Biol. Chem. 271: 19402-19408, 1996.
2. Wang, Q. et.al: Cell cycle arrest and apoptosis induced by human polo-like kinase 3 is mediated through perturbation of microtubule integrity. Molec. Cell. Biol. 22: 3450-3459, 2002.

Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Molecular Weight :Observed MW ~35 kDa. Calculated MW ~34.3 kDa.

Product Sheets (By Lot #) :

T4229-6.pdf

Research Areas :Cell Cycle, Cellular Stress, Ser/Thr Kinases