PI3K (p110 alpha (E545K)/p85 alpha), Active(P27-152G)

PI3K (p110 alpha (E545K)/p85 alpha), Active(P27-152G)

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Description :Recombinant full-length human p110alpha (E545K) mutant subunit and full-length human p85alpha wild-type subunit were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.

Species :Human

Tag :GST

Expression System:Sf9 insect cells using baculovirus

Sequence :Full length

Genbank Number :NM_006218

Genbank Number 2 :NM_181523

Alternative Name(s) :p110alpha: PIK3CA, MGC142161, MGC142163, p110-alpha p85-alpha: PIK3R1, GRB1, p85-ALPHA

Formulation :Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.

Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background :The PI3K comprises of a 110 kDa catalytic subunit and an 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110 catalytic subunit (PIK3CA) is frequently mutated or amplified in a variety of cancers including ovarian and colon and this protein is one of the PI3K mutants (1). PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of p110 is essential for vascular development and inactivation of p110 leads to severe defects in angiogenic sprouting and vascular remodeling (2).

References :1. Samuels, Y. et al: High frequency of mutations of the PIK3CA gene in human cancers. Science 304: 554 only, 2004.
2. Graupera, M.et al: Angiogenesis selectively requires the p110-alpha isoform of PI3K to control endothelial cell migration. Nature 453: 662-666, 2008.

Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Molecular Weight :p110α(E545K):
Calculated MW ~148 kDa.

p85α:
Calculated MW ~108 kDa.

Product Sheets (By Lot #) :

J3786-11.pdf

J4064-7.pdf

Research Areas :Cancer, Cell Cycle, Receptor Tyrosine Kinases