PI3K (p110 alpha(H1047R)/p85 alpha), Active(P27-122EH)
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Description :Recombinant full-length human p110 (H1047R) and p85subunits were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Species :Human
Tag :HIS
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_006218
Genbank Number 2 :NM_181523.
Alternative Name(s) :p110 alpha: PIK3CA, MGC142161, MGC142163, p110-alpha p85 alpha: PIK3R1, GRB1, p85-ALPHA
Formulation :Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
Storage and Stability :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Scientific Background :The PI3K comprises of a 110 kDa catalytic subunit and an 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110 catalytic subunit (PIK3CA) is frequently mutated or amplified in a variety of cancers including ovarian and colon and this protein is one of the PI3K mutants (1). PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of p110 is essential for vascular development and inactivation of p110 leads to severe defects in angiogenic sprouting and vascular remodeling (2).
References :1. Samuels, Y. et al: High frequency of mutations of the PIK3CA gene in human cancers. Science 304: 554 only, 2004.
2. Graupera, M.et al: Angiogenesis selectively requires the p110-alpha isoform of PI3K to control endothelial cell migration. Nature 453: 662-666, 2008.
Specific Activity :Sample Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Molecular Weight :p110 alpha
Calculated MW ~124 kDa.
p85 alpha
Calculated MW ~85 kDa.
Product Sheets (By Lot #) :
Research Areas :Cancer, Ser/Thr Kinases