Anti-PACRG(P216-363R)
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Description :Rabbit Polyclonal Antibody
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Specificity :Recognizes the human PACRG protein
Cited Applications :ELISA, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human, Mouse and Chicken
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against synthesized peptide corresponding to amino acids 204-215 of human PACRG protein
Purification :Immunoaffinity chromatography
Stability :1yr at –20oC from date of shipment
Sample Data :Western blot using affinity purified Anti-PACRG (1:1,500 dilution) shows detection of a band ~40 kDa corresponding to human PACRG (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Load: ~ 35 ug of a mouse embryonic fibroblast (MEF) whole cell lysate.
Scientific Background :PACRG (also known as Parkin coregulated gene protein and PARK2 coregulated) is a gene located very close to parkin, in reverse orientation on the chromosome. It is thought to be co-transcribed with parkin by a bi-directional promoter between the two genes. PACRG is expressed in all immune tissues, spleen, lymph nodes, thymus, tonsils, leukocyte and bone marrow and is also expressed in heart, brain, skeletal muscle, kidney, lung and pancreas. PACRG is expressed in primary Schwann cells and very weakly by monocyte-derived macrophages, which are the primary host cells of Mycobacterium leprae, the causative agent of leprosy. Splice variants have been described for this protein.
References :
1. Mira,M.T., et al.: Susceptibility to leprosy is associated with PARK2 and PACRG. Nature. 2004: 427 (6975); 636-640.
2. Imai,Y., et al.: A product of the human gene adjacent to parkin is a component of Lewy bodies and suppresses Pael receptor-induced cell death. J. Biol. Chem. 2003: 278 (51); 51901-51910.
3. West,A.B., et al.: Identification of a novel gene linked to parkin via a bi-directional promoter. J. Mol. Biol. 2003: 326 (1); 11-19.
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