Anti-phospho-Parkin (Ser101)(P215-65R)
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Description :Rabbit Polyclonal Antibody
Species :Rabbit
Tag :
Expression System:
Sequence :
Specificity :Recognizes the Parkin protein phosphorylated at serine 101
Cited Applications :WB
Cross Reactivity :Human, Bovine and non-Human Primate
Host :Rabbit, IgG
Immunogen :Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser101 conjugated to KLH
Purification :Affinity Chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot of HEK293 cells transfected with Parkin WT (Phospho) and Parkin S101 mutant (non-phospho) showing the phospho-specific immunolabeling of the ~ 52 kDa Parkin protein. The immunolabeling is absent in the Parkin S101 mutant.
Scientific Background :The Parkin protein is a component of the E3 ubiquitin ligase complex, which is responsible for targeting of proteins for degradation. Hereditary Parkinson’s disease is most commonly caused by mutations in the parkin gene. The disease is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra (1). Recent studies indicate that phosphorylation of Parkin at Ser101 and Ser378 might be important for regulating E3 ubiquitin ligase activity (2).
References :
1. Jenner P. et al: Oxidative stress as a cause of nigral cell death in Parkinson’s disease and incidental Lewy body disease. Ann Neurol. 32 Suppl: S82-7, 1992.
2. Yamamoto A. et al: Parkin phosphorylation and modulation of its E# ubiquitin ligase activity. J Biol Chem. 280(5):3390-9, 2005.
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Research Areas :Apoptosis/Autophagy, Cancer, Cell Cycle, Neurobiology, Cancer, Neurobiology, Apoptosis/Autophagy, Cell Cycle