FIP1L1-PDGFR alpha, Active(P12-19BG)
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Description :Recombinant human FIP1L1-PDGFRA, the fusion protein [FIP1L1 (1-265)-PDGFRA (579-end)], was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :1-265 (FIP1L1)
Genbank Number :AM392758
Genbank Number 2 :NM_006206
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay
Molecular Weight :130 kDa
Gene Aliases :FIP1L1: FIP1, RHE, DKFZp586K0717
PDGFRA: CD140A, PDGFR2, MGC74795, Rhe-PDGFRA
Scientific Background :FIP1L1 or factor interacting with PAPOLA and CPSF1 is a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that transforms hematopoietic cells and is inhibited by imatinib in which fusion of the PDGFRA and FIP1L1 genes as a therapeutic target of imatinib in idiopathic hypereosinophilic syndrome (1). FIP1L1-PDGFR-alpha requires disruption of the juxtamembrane protein of PDGFR-alpha and is FIP1L1-independent (2).
References :
1. Cools, J. et.al: A tyrosine kinase created by fusion of the PDGFRA and FIP1L1 genes as a therapeutic target of imatinib in idiopathic hypereosinophilic syndrome. New Eng. J. Med. 348: 1201-1214, 2003.
2. Stover, E. H. et.al: FIP1L1-PDGFR-alpha requires disruption of the juxtamembrane protein of PDGFR-alpha and is FIP1L1-independent. Proc. Nat. Acad. Sci. 103: 8078-8083, 2006.
Product Sheets (By Lot #) :
Research Areas :AKT/PKB Pathway, Cancer, ERK/MAPK Pathway, JAK/STAT Pathway, Receptor Tyrosine Kinases, Cancer, AKT/PKB Pathway, ERK/MAPK Pathway, JAK/STAT Pathway, Receptor Tyrosine Kinases