Anti-phospho-PAK1/2/3 (Thr423)(P02-653R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the PAK (P21-GTPase Activated Protein Kinase)
Cited Applications :ELISA, WB
Ideal working dilutions for each application should be empirically determined by the investigator.
Cross Reactivity :Human
Host Isotype / Clone# :Rabbit, IgG
Immunogen :The antibody was produced against human PAK1/2/3 phospho peptide corresponding to a region in the protein conjugated to KLH.
Purification :Immunoaffinity chromatography
Stability :1yr at –20oC from date of shipment
Scientific Background :In mammals, there are several identified isoforms of p21-activated protein kinases or PAKs. PAKs 1, 2 and 3 are members of the serine/threonine p21-activating kinases that serve as targets for the small GTP binding proteins Cdc42 and RAC1. They have been implicated in wide range of biological activities, which include cytoskeletal reorganization and nuclear signaling. CDC42 and RAC1 induce autophosphorylation of PAK2, which stimulates sustained phosphorylation of other substrates (2). Autophosphorylation of Thr402 is essential for activation of PAK (Jakobi et al., 2000).
References :1. Martin, G. A. et.al: A novel serine kinase activated by rac1/CDC42Hs-dependent autophosphorylation is related to PAK65 and STE20. EMBO J. 14: 1970-1978, 1995. 2. Jakobi R. et al: Substrates enhance autophosphorylation and activation of p21-activated protein kinase gamma-PAK in the absence of activation loop phosphorylation. Eur J Biochem 267:4414-4421, 2000.
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Research Areas :Cancer, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Neurobiology, Ser/Thr Kinases