Anti-phospho-PAK1/2/3 (Thr402)(P02-652R)
FOR BULK ORDER REQUESTS PLEASE CONTACT US
Description :Rabbit Polyclonal Antibody
Species :Rabbit
Tag :
Expression System:
Sequence :
Specificity :Recognizes the PAK1/2/3 protein phosphorylated at threonine 402
Cited Applications :WB
Cross Reactivity :Human, Mouse, Rat, Bovine and Canine
Host :Rabbit, IgG
Immunogen :Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr402 conjugated to KLH
Purification :Affinity Chromatography
Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68kDa to ~70kDa PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-PAK1/2/3 (Thr402) antibody. The immunolabeling of PAK is completely eliminated by treatment with lambda-Ptase.
Scientific Background :In mammals, there are several identified isoforms of p21-activated protein kinases or PAKs. PAKs 1, 2 and 3 are members of the serine/threonine p21-activating kinases that serve as targets for the small GTP binding proteins Cdc42 and RAC1. They have been implicated in wide range of biological activities, which include cytoskeletal reorganization and nuclear signaling. CDC42 and RAC1 induce autophosphorylation of PAK2, which stimulates sustained phosphorylation of other substrates (2). Autophosphorylation of Thr402 is essential for activation of PAK (Jakobi et al., 2000).
References :
1. Martin, G. A. et.al: A novel serine kinase activated by rac1/CDC42Hs-dependent autophosphorylation is related to PAK65 and STE20. EMBO J. 14: 1970-1978, 1995.
2. Jakobi R. et al: Substrates enhance autophosphorylation and activation of p21-activated protein kinase gamma-PAK in the absence of activation loop phosphorylation. Eur J Biochem 267:4414-4421, 2000.
Product Sheets (By Lot #) :
Research Areas :Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Inflammation, JNK/SAPK Pathway, Metabolic Disorder, Neurobiology, p38 Pathway, Cancer, Neurobiology, Inflammation, ERK/MAPK Pathway, Invasion/Metastasis, Ser/Thr Kinases