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Nodal(N822-40N)

Nodal(N822-40N)

  • $501.00


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Description :Recombinant Human Nodal was expressed in E. coli cells.

Species :Human

Tag :

Expression System:E.coli

Sequence :

Genbank Number :Q96S42

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/mu;g of recombinant protein as determined by the LAL method

Molecular Weight :13.1 kDa

Gene Aliases :Nodal homolog

Reconstitution Protocol :Reconstitute in sterile 4mM HCl at 100μg/mL (containing at least 0.1% human or bovine serum albumin)

Scientific Background :Nodal, also known as Nodal homolog, is a secreted protein member of the Transforming Growth Factor-beta (TGF-beta) superfamily. It is essential for the formation of mesoderm and axial patterning during early vertebrate embryonic development.

Sample Cell Assay Data :100 ng/mL phosphorylation activity as determined by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells.

Purity :Recombinant Human Nodal resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250

Product Sheets (By Lot #) :

G3223-1.pdf

Research Areas :Cancer