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Description :Recombinant full-length human NNMT was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :Full length
Genbank Number :NM_006169
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability, and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Methyltransferase Assay
Molecular Weight :54 kDa
Gene Aliases :None
Scientific Background :Nicotinamide N-methyltransferase (NNMT) is expressed predominantly in the liver, and mediates the N-methylation of nicotinamide and other pyridines. The activity of NNMT is involved in drug and xenobiotic toxicity (1). NNMT has also been identified as serum tumor marker for colorectal cancer (2), as well as potential marker in urine-based diagnostic test for bladder cancer (3).
1. Aksoy, S., et al. Human liver nicotinamide N-methyl-transferase. cDNA cloning, expression, and biochemical characterization. J Biol Chem. 269(20):14835-40, 1994.
2. Roessler, M., et al. Identification of nicotinamide N-methyl-transferase as a novel serum tumor marker for colorectal cancer. Clin Cancer Res. 11(18):6550-7, 2005.
3. Sartini, D., et al. Upregulation of tissue and urinary nicotinamide N-methyltransferase in bladder cancer: potential for the development of a urine-based diagnostic test. Cell Biochem Biophys. 65(3):473-83, 2013.
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