NLK, Active(N15-10G)

NLK, Active(N15-10G)

  • $226.00


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Description :Recombinant full-length human NLK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :NM_016231

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Western Blot

Molecular Weight :~84 kDa

Gene Aliases :FLJ21033, DKFZp761G1211

Scientific Background :NLK (also known as nemo-like kinase) is a member of the serine/threonine protein kinase that is encoded by the NLK gene. NLK plays a role in cell fate determination and is required for the differentiation of bone marrow stromal cells. NLK also suppresses a wide range of transcription factors such as CREB by phosphorylation of the co-activator CREBBP. NLK expression increases the number of apoptotic cells but does not induce obvious cell cycle arrest (1). NLK suppresses Notch signalling by interfering with formation of the Notch active transcriptional complex (2).

References :
1. Yasuda, J. et.al: Nemo-like kinase induces apoptosis in DLD-1 human colon cancer cells. Biochem. Biophys. Res. Commun. 308: 227-233, 2003.

2. Ishitani T, et.al:. NLK suppresses the Notch signalling pathway. Nat Cell Biol, 2010 Mar.

Product Sheets (By Lot #) :

I226-2.pdf

Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases, Cancer, Cell Cycle, Ser/Thr Kinases