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NEK1, Active (N02-18G)

NEK1, Active (N02-18G)

  • $226.00


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Description :Recombinant human NEK1 (1-477) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :1-477

Genbank Number :NM_012224

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~85 kDa

Gene Aliases :ALS24; SRPS2; SRTD6; SRPS2A; NY-REN-55

Scientific Background :NEK1 or NIMA (never in mitosis gene a)-related kinase 1 is a serine/threonine kinase involved in cell cycle regulation and is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. NEK1 is involved early in the DNA damage response pathway (1). NEK1 cycles through the nucleus via its nuclear localization and export signals (2). NEK1 protein participates in different signaling pathways to regulate diverse cellular processes and plays an important role in the kidney where it has opened a new avenue for studying cystogenesis and identifying possible modes of therapy.

References :
1. Polci, R. et.al: NIMA-related protein kinase 1 is involved early in the ionizing radiation-induced DNA damage response. Cancer Res. 64: 8800-8803, 2004.

2. Hilton, L. K. et.al: The NIMA-related kinase NEK1 cycles through the nucleus. Biochem. Biophys. Res. Commun. 389: 52-56, 2009.

Product Sheets (By Lot #) :

E3332-13.pdf

Research Areas :Cancer, Cell Cycle, Ser/Thr Kinases