Anti-NOTCH 2(N42-363R)

Anti-NOTCH 2(N42-363R)

  • $695.00


Description :Rabbit Polyclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the NOTCH 2 protein

Cited Applications :WB, ELISA, IHC

Cross Reactivity :Human

Host / Isotype / Clone# :Rabbit, IgG

Immunogen :This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human NOTCH 2 located near the N-terminal sequence of the cleaved intracellular domain (NICD).

Purification :Affinity chromatography

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Anti-NOTCH 2 was diluted 1:500 to detect NOTCH 2 in human liver tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Scientific Background :The Anti-NOTCH 2 antibody recognizes NOTCH 2 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called NOTCH extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

References  :
1. Bertrand FE, Eckfeldt CE, Lysholm AS, LeBien TW, Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells. Leukemia. 12: 2095-102. 2000.

2. Gridley T , Notch signaling and inherited disease syndromes. Hum. Mol. Genet.: 12, Suppl 1, R9-R13. 2003.

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Research Areas :Cancer, Neurobiology, Metabolic Disorder, WNT Signaling