TFG-TRKA (TRK-T3), Active(N16-19CG)
FOR BULK ORDER REQUESTS PLEASE CONTACT US
Description :Recombinant human fusion TFG (1-193)-TRKA (399-end) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Species :Human
Tag :GST tag
Expression System:Sf9 insect cells using baculovirus
Sequence :1-193 (TFG)-399-end (TRKA)
Genbank Number :NM_001195478
Genbank Number 2 :NM_002529
Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.
Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Applications :Kinase Assay
Molecular Weight :~100 kDa
Gene Aliases :TFG: HMSNP; SPG57; TF6
TRKA: NTRK1, MTC, TRK, p140-TrkA
Scientific Background :TFG-TRKA (TRK-T3) is a chimeric cytoplasmic oncoprotein isolated from a papillary thyroid tumor. This oncoprotein is the consequence of a t(1, 3) translocation juxtaposing the tyrosine kinase domain of the NTRK1 receptor to the N-terminal portion of a protein encoded by TFG (TRK-fused gene), a novel gene first discovered in the rearranged form. TRK-T3 fusion oncoprotein displays a constitutive tyrosine kinase activity resulting in its capability to transform mouse NIH3T3 cells. The TFG portion of TRK-T3 contains a coiled-coil domain most likely responsible for the constitutive, ligand-independent activation of the receptor tyrosine kinase activity through TRK-T3 oligomerization.
References :
1. Greco A. et al: The DNA rearrangement that generates the TRK-T3 oncogene involves a novel gene on chromosome 3 whose product has a potential coiled-coil domain. Mol Cell Biol. 15(11):6118-27, 1995.
2. Greco A, et al. Role of the TFG N-terminus and coiled-coil domain in the transforming activity of the thyroid TRK-T3 oncogene. Oncogene. 12;16(6):809-16, 1998.
Product Sheets (By Lot #) :
Research Areas :Cancer, Neurobiology, Receptor Tyrosine Kinases, Cancer, Neurobiology, Cardiovascular Disease, AKT/PKB Pathway, JAK/STAT Pathway, Angiogenesis, Ser/Thr Kinases