Anti-Neurofilament-L (NF-L)(N125-561M)

Anti-Neurofilament-L (NF-L)(N125-561M)

  • $695.00


Description :Mouse Monoclonal Antibody

Species :

Tag :

Expression System:

Sequence :

Specificity :Recognizes the Neurofilament-L (NF-L) protein

Cited Applications :WB, IF

Cross Reactivity :Human, Mouse, Rat, Bovine and Chicken

Host / Isotype / Clone# :Mouse, IgG, DA2

Immunogen :Preparation of porcine intermediate filaments

Purification :Unpurified, concentrated culture supernatant

Stability :Store at 4oC (add 0.1% NaN3) for several months, and at -20oC for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Sample Data :Western blot of rat cortex lysate showing specific immunolabeling of the ~68k NF-L protein.

Scientific Background :Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (2).

References :
1. Harris, J., Ayyub, C. and Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62. 1991.

2. Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. Am. J. Hum. Genet. 67:37-46. 2000.

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Research Areas :Neurobiology