Anti-NF-kB p105(N12-63R)
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Description :Rabbit Polyclonal Antibody
Species :
Tag :
Expression System:
Sequence :
Specificity :Recognizes the NF-κB p105 protein
Cited Applications :WB
Cross Reactivity :Western blot of human and mouse NF-kB p105
NF-kB p105 from other species may also be detectable.
Host :Rabbit
Immunogen :Synthetic peptide corresponding to amino acids 930-934 of human NF-κB p105
Purification :Affinity chromatography
Stability :Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Sample Data :Representative western blot with Anti-NF-κB p105 (1:1000) using 30 μg of HeLa, HEK-293T, and Jurkat cell lysate.gel.jpg)
Scientific Background :Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses. In unstimulated cells, NF-κB is sequestered in the cytoplasm by its inhibitory proteins, the IκBs (1). NF-κB-activating agents can induce the phosphorylation of IκB’s, targeting them for rapid degradation through an ubiquitin-proteasome pathway, releasing NF-κB to enter the nucleus, where it regulates gene expression.
References :
1. Baeuerle, P. et al: Function and activation of NF-kappa B in the immune system.Annu Rev Immunol. 1994;12:141-79.
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Research Areas :Cancer, Inflammation, AKT/PKB Pathway, p38 Pathway, Apoptosis/Autophagy