MYLK3, Active(M64-10G)

MYLK3, Active(M64-10G)

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Description :Recombinant full-length human MYLK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species :Human

Tag :GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence :Full Length

Genbank Number :BC109097

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight :~135 kDa

Gene Aliases :caMLCK; MLCK, MLCK2, MGC126319, MGC126320

Scientific Background :MYLK3 (or myosin light chain kinase 3) plays a key role in rate of cross-bridge recruitment in cardiac myocytes. MYLK3 is upregulated in failing human myocardia and expression of MYLK3 correlates with the pulmonary arterial pressure of patients with heart failure (1). Knockdown of MYLK3 with small interfering RNAs in cultured rat cardiomyocytes decreases Myl2 phosphorylation and impairs epinephrine-induced activation of sarcomere reassembly (2). Recombinant murine MYLK3 shows specificity for Myl2 (Mlc2v) and the phosphorylation is calcium- and calmodulin-dependent.

References :
1. Chan, J. Y. et al:Identification of cardiac-specific myosin light chain kinase. Circ. Res.2008; 102: 571-580.

2. Seguchi, O. et al: A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart. J. Clin. Invest. 2007;117: 2812-2824

Product Sheets (By Lot #) :

E117-1.pdf

I124-1.pdf

I208-4.pdf

Research Areas :Cardiovascular Disease, PKA/PKC Pathway, Ser/Thr Kinases, Cardiovascular Disease, PKA/PKC Pathway, Ser/Thr Kinases