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Description :Recombinant Human MSTN was expressed in E. coli cells.

Species :Human

Expression System:E.coli

Genbank Number :O14793

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method.

Molecular Weight :13.0 kDa

Gene Aliases :MSTN, Growth differentiation factor 8, GDF-8, Recombinant Human Myostatin (MSTN)

Reconstitution Protocol :A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Scientific Background :Growth differentiation Factor 8 (GDF-8), also known as myostatin, is a secreted protein that is expressed specifically in developing and adult skeletal muscle. It controls myoblast proliferation and is a potent negative regulator of skeletal muscle mass. GDF-8 belongs to the transforming growth factor beta (TGF-beta) superfamily, which includes the TGF-betas, bone morphogenetic proteins (BMPs), growth differentiation factors (GDFs), activins, inhibins, leftys, nodal, Mullerian inhibitory substance (MIS) and the glial cell line-derived neurotrophic factors (GDNFs). All TGF-β superfamily members are synthesized and secreted as a homodimeric prepropeptide that is cleaved by proprotein convertases such as furin to generate the dimeric N-terminal propeptide and the dimeric C-terminal mature active protein.

Sample Cell Assay Data :The ED(50) was determined by the dose-dependent proliferation inhibition of human MPC-11 cells was found to be in the range of 20-40 ng/mL.

Purity :Recombinant Human MSTN resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250

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Research Areas :Cancer