Midkine (NEGF2)(M838-40N)

Midkine (NEGF2)(M838-40N)

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Description :Recombinant Human Midkine (NEGF2) was expressed in E. coli cells.

Species :Human

Expression System:E.coli

Genbank Number :P21741

Storage, Stability and Shipping :The lyophilized protein is stable for at least one year from date of receipt at -70oC. Upon reconstitution, this cytokine can be stored in working aliquots at 2o - 8oC for one month, or at -20oC for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Applications :Cell Assay

Endotoxin Level :<1.0 EU/μg of recombinant protein as determined by the LAL method.

Molecular Weight :14.0 kDa

Gene Aliases :MK, NEGF-2

Reconstitution Protocol :Reconstitute at 0.1 mg/ml in sterile PBS

Scientific Background :Midkine and its functionally-related protein Pleiotrophin (PTN), are heparin-binding neurotrophic factors that signal through the same receptor. Midkine is involved in a number of biological processes including the migration of neutrophils, inflammatory leukocytes, macrophages, neuronal cells, and osteoclasts. Midkine is a basic, non-glycosylated polypeptide that contains five intra-chain disulfide bonds. There is 87% identity between the human and murine Midkine and approximately 50% identity between human Midkine and human PTN with the conservation of all 10 cysteines.

Sample Cell Assay Data :The ED (50) was determined by the dose-dependent proliferation of AsPC-1 cells and was found to be <0.5ng/mL.

Purity :Recombinant Human Midkine (NEGF2) resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250

Product Sheets (By Lot #) :

G3211-94.pdf

Research Areas :Cancer